Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTVAAPAPALAEVACFSRDL-------CKE-LVKEWSSLDSSCFSISTVSGGITNMLLKVSVKEG----SDSESSVTVRLYGPNTDLVIDRERELQGSGLGYLEYLRMEWFRHSSMLGHYHLQVYTQRLLFCTHRCMETGEPAINASANNMKEPRIAAEIAKQLQKFHQVDIPGSKEPQ-LWNDIFKFLKKASVLKFEDNEKQKRYETISFREIQDEVKELKDLSDLLHAPVVFAHNDLLSGNLMLNDLEEKLYFIDFEYGSYSYRGFDIANHF---------NEYAGFECDYNLYPDKDVQYHFFRNYLSDRPSELKNRLFASICEKRNVGYLAFVQYKVQEQDLDDLYTETNTFRLASHLYWALWALIQARVSPIDFDYLGYFFLRRHVVPNRKLYNL 2IG7 Chain:A ((49-379)) ------------------RDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAI----------LQGVDSLVLESVMFAILAERSL----GPQLYG---VFPEGR-LEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLP------MNLLEMYS---LKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLS----LMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLA----EAKKGETLSQEEQR----------KLEE----DLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDY----------------

General information: TITO was launched using: RESULT: Template: 2IG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1289 23201 18.00 78.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 18.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_2IG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ig7-query.scw PDB file : Tito_Scwrl_2IG7.pdb: