Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELGNFQKSVDSDEIKTMAFEIIGEPVKAVSRICEQWGEAEDEVREYDVYVIETDAGKYTLKKTGS--------KEAQIYAQYLSK--GEFHVPQYVGMRQAEDADWICMKYVEGNDMRDMT---------DETTEKAAETLSKIQSYFWTPSMDKAPENEVEQRFVEYWKRVLR---RASS-VADD-PILRKAYQMFLD--RQLTCPCTMSNGDFLQWNVIYDG-ENV-VMIDWGFGGMMPYPLDIARFLAHATETRSTFPFYMNDAQKELFLDRMYEALKTKISREQFNLDVKLATLNEYIEFVEAEEDEDGWYLEHAQALAEELLNL 5IQI Chain:C ((9-303)) --------ATNVKAMKYLIEHYF-----DNFKVDSIE--IIGSGYDSVAYLVN---NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC----TIDNK-QNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEI-------GTNFGEDILRMYGNID--IEKAKEYQDIVEEYYPIETIVYGIKNIK-QEFIENGRKEIYKRTY--

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1164 4959 4.26 18.57 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 4.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: