Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GCSASILKYDANGGQETYYGSALPANGSIAFQPTEFGTEVVISVDANCNPTNTDDVKKSVPKGWGLH----- 2MM2 Chain:A ((1-65)) NCTANILNIN-----EVVIATGCVPAGGNLIIRVGSDHSYLIRATVSCGLSLNPS--QSFINGESLASGGRC

General information: TITO was launched using: RESULT: Template: 2MM2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 251 -22646 -90.22 -377.43 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -90.22 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2MM2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2mm2-query.scw PDB file : Tito_Scwrl_2MM2.pdb: