Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTYFELSDDFENIIKNTLNDKQIISIKQNPTGWTNIVYEVETNEGNFFFRFPRDEFWSRTIVKDYEFAKYIYGK--TNFDTVKLELFYDN-DRPFSVHKKISGIPLAEVMNDL-SSDEVKTISDEIAKFMYQLHCLDFKESK-----IFSINNIGLNLTDF-LDELLNVH---VDKNDMNFWIPTAIHSDDICLVHGDLNSSNVLLDENRHVAAIIDFGFGGFGNKYNDIGRIIGR----CPTSFKNEIINSYEQY-SNSKLDSKLLDTNINTWTDIDNSYINYMRTIGIYK 5IQI Chain:C ((10-266)) ------TNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLV---NNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEY----------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 964 -7359 -7.63 -30.79 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -7.63 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: