Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLDNSDKYLLPLLPKIILEKTNKK-AEKIKFIGGGSFGRVYKAELSNGETIAIKAYRI---QGSQYEEANQLNLLSKNTSVKTPKVIFTYEDE---NTAILAMTFVEGKNVLNPLFLF---KSKKQKQLFANDVVDGMLQWHSVTGEKFGALSNPTYDSWYEYYKTVNQELWLKDLAKLAKNGKFSKKRMQLLYDATEIFNKLPEETTAPVLIHSDLNIMNIMADPKTLKLTAFIDPCTSIWADREYDLFQLRNMW-----GDAYGLYETYKKKYKLSKYADFRVAYYGAMLEASMRLKGGLI-LPFWEDLTMMRLKKEIKMLNNL 3N4U Chain:A ((3-297)) --------TYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI----NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLT---PLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNL---VLDFREKINEDNKKIKK-LLSREL-KGPQMKKVDDFYRDILENEI--YF-KYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPT--VLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIRS--------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1222 48036 39.31 172.17 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 39.31 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: