Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSAENLSQMGAANVQR------------------LVDQYGQQVIEKCPVSP------------VEYAFYERAA-----EELNSRG----IKTPT-----LLSADPHPRRLRLEYIPGKVSQDEVS-GDDILKCLSH----LHHTPPD------PTWVYH------------PHSWPEKALEKSLALLRLPDRAAHQMRCFQQAGSVLFRQKCLISGDTN--AGNWGR-----------RENGEWV-LFDWE-RFGTG----------------SPAIDLAPLVK------GMGSRQSILQLAERYSVHC---PHMPARQLATEITLAKAWIVSEVM---SLLYDRN-----------KTDFELYLNWYRETLPGWLDEAISVL- 4RAP Chain:A ((10-391)) ITPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVRLLDADSENILFCCDVDKGWVTSSKKYFVRFRIQVFRQGATPLLDETLKLKDRPVLISFPTGTLGDLLGWFPYAERFQSLHK----CRLECTMSQDIIDLLAPQYPQIQFSTPDKPRTVAPYATYRVGLYFGGDTNNQPVDFRKVGFHRSAGYILGVDPREAPVRLDLSAPRVIAAPYVCIATQSTCQAKYWNNGTGWSEVIAHLKSLGYRVMCIDRDAHYGQGFVWNHIPWGAEDFTGKLPLQERVNLLRHASFFIGLPSGLSWLAWATRIPVVLISGFSLPNSEFYTPWRVFNSHGCYGCWDDTSLNFDHHDFLWCPRHKNTDRQFECTRLITGAQVNGVINKLHRSLT

General information: TITO was launched using: RESULT: Template: 4RAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1136 15943 14.03 61.79 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 14.03 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.123

(partial model without unconserved sides chains): PDB file : Tito_4RAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4rap-query.scw PDB file : Tito_Scwrl_4RAP.pdb: