Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAPTEEMISVQKLVERLNAEYAFE-VDYIGADLGGFSSQNNQLTLKDKRRMVLKRCDQSQW--DP--RNVDIATILAH-HSSLIYAPLATVGGSSTFKF----DGWVYAVFPYVDGEKLHQHSLDAETLKSAARALYAFHHIAHFAGSHAA-QRPNVS--DF-EREV---TSVLEPTLSLSTDHADIIIRSIEAKRHILHSFSSDLSSINVGASSVIHGDFHNENILYRSD---HVVAILDFEMLHWGNVEDDIISFIALGCCNNGFSEENLRMANIFVKEYLSLHGENLDLHTALLRFIHLKSSSLFLQKMLIYTGNMLYAQIIERDNKLFPSIMKKHREISKTLSSL 6EF6 Chain:A ((27-303)) ----------DVTIAQQALTHYDVSDNASLRLL-NLSENATYLVEDGEH-QSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQR-WTRPAGFGRFSWDWEHCLGDTPRWGRWQD-AEGVG-ASE---TALLTRAQDLLHRKLEEYG-SGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDP-R---LGEWQESWVAGYRSRRELPAADEAML-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1116 -4922 -4.41 -19.38 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -4.41 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: