Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFENESMGFNHDEICATWNIPPLVSVQKPLTGTIHNVFLLTT-REA-KYVLRIYSYPPEKRFRIVNEHAIARYVQSHHLP-AIAPLPISSGCETFLERDGHFYALYPFAQGEQLSRAMLNSNQSHVGEIISAMGHSLAEVHLVLSSYCLPTTRPLSLVVNREQTIAKIEELEIVI---PA---KEALDDLD----QRVLSALRARKQYLLTASDVD-VSELNALPWQPLHGDFQETNLFFSHGRVSAIIDWDQACSGPRAWEILRTLHYVFAL---DPSRCQRFLEAYQQVFPLPMEVLHLTAKVYTWVQLNNLWGWRSYYLDNNQSLRQLLSTSDSQPFTTRWAQLAKML 4PDY Chain:A ((23-316)) ----------IPQAVVSKYDL-AIQQR---H---ADGNIEVWTDSKGRRYAAKRSSIA-PAH--CRIMVQCLRHAQEQGFTKFARFVTTSSNA-PYVRHGDFTYYVTEWVSGQPANF--GLPEH------VAQTAYTLAQFHEATRSFRTDWK----DDV-FGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLAL-FEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDAVRNIPKSEYRAVEAI-LR-FPYLPWRIAHARYHFA--------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1230 -52020 -42.29 -191.25 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -42.29 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: