Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSINFSNLS--------KNINFINFSKISRGNNKELKYIVKDSEMNRYLFKINSIIHLDNKVNEFRFAKRIEHLPFEK---SEIVDLCVFNQNRYICSIYKWIDGK-----ILED----QIKNYPSNKQYSFGFKAGRSLKLI--------------HDLPIRDEELKQVEELPVRKIIEYYHQNK--------RFENDNIIIDFILKNNSKIKD-EKICFLHGHYSIKN----FIIKNNDEIS--------LIDYNQCYFGDYVQDFSKNEIYNSYYSEQFAQGII-------------DGYFYNRKIP----DTFWLKYAYYAAYNC-----ITLFLWASKQEVISTKIKIKEMIQNVLNQFNNFESI--IPNWYKKNVLSK----ERQKFINSPYTLEKILGT-------PKKENKNDSKIKYFDEMYDDNDDDLLDEKMMDSFKEFLKNVAKK 2BMF Chain:A ((169-618)) MASIEDNPEIEDDIFRKKRLTIMDLHPGAGKTKRYLPAIVREAIKRG----LRTLILAPTRVVAAEMEEALRGLPIRYQTGREIVDLMC--HATFTMRLLSPIRVPNYNLIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEE-REIPERSWNSGHEWVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYIKTRTNDWDFVVTTDISEMGANFKAERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRVGRNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGVKNNQILEENVEVEIWTKEGERKKLKPRWLDARIYS------DPLALKEFKEFAAGRK--

General information: TITO was launched using: RESULT: Template: 2BMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1819 43355 23.83 123.17 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 23.83 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_2BMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2bmf-query.scw PDB file : Tito_Scwrl_2BMF.pdb: