Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MEMRGGDLEAAALALWPDLARQMG------EEAGLWR-----AAPLARREDARVARVLLRLDGPEGRRLVLKHQAR-------PVVPDKFADGIAAHVAVQEVWPEGLPVLQAVDLERQACVMEYL---PARPLSVLLEGAPLET----------QAALLRRAGAWMDG----FHRALHGEARVFQPKFTIHFLRSVMGELRAGERRVAEPERFLVCAEALCAM--QPDFEGQETVTAQTHGDLHQRNLV---LDETRCWGVDFAGGRVV--PVGHDIARLLTDYAILHAPKEAIPKGQVLPPQAQSAFFEGYGLVGPGDPSVQLLLRNRV--------LAEWWGLPAREAERSRAQARRFGRLMALVDRVFG----- 4D0P Chain:A ((16-386)) NLYFQGGG--RMAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPNLPDSTAKEIY-HFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYA--RVLDYRRKFIEAAQRYNELSYKTIVHESERLEALKHA-LHCTILASAGQQRSRMLATLFKDERCQQLAAYGILEKMYLDRIIRGNQLQEFAA--MLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIPAAKAEKIASQMITEGRMNGFIDQIDGIVHFE

General information: TITO was launched using: RESULT: Template: 4D0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1323 -20855 -15.76 -66.21 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -15.76 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_4D0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4d0p-query.scw PDB file : Tito_Scwrl_4D0P.pdb: