Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEEFANRINLNTDLSNISKIICNKYNLGEYISDAVITVGYEDFNYTLETT-QG-KYCVKVFNKERTDIECEKYIERIELASKLNIN-TPKLYRVDEKSECNIKLENIKYRLCVFDYIDGKSFYDLGKIPNQDEINEIIRQMAIIHKATLQSDFIYDKWAIINFV-------KEFQNKK---------QYL-------NGNDYKKLEELYNRFLQV------DIEKLPKAFVHGDIISTNVMKDKNNKLWIIDFAVSNYVPRIIDLAVSSCNLCLDTENKENTKKKVKMILEEYQKYNKLTDYELEQFPLFFDIANAMGILQISHLNTLGELSEEDKFWYDESEKGLEFSNEEFWKDIYVKK 4PDY Chain:A ((23-315)) -----------------IPQAVVSKYDLAI----QQR---HADGNIEVWTDSKGRRYAAKRSSI--APAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVR-HGD--FTYYVTEWVSGQPANFG----LPEHVAQTAYTLAQFHEATRSFRTDWK----DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERG-NW--DGHLAYACFLHFDAVRNIPKSEYRAVEAILRFPYLPWRIAHARYHF----------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1070 -3145 -2.94 -12.29 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -2.94 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: