Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------KGCPYSIKRYGEEVKWDIIEAGTS-HIRVVN-GIGATIIAK-------KNCKLSLKGLPRFEYTIS---------------- 3D33 Chain:A ((34-134)) PVKDVELDGRWDDNCPITVFTDGYLLTLKNASPDRDMTIRITDMAKGGVVYENDIPEVQSAYITISIANFPAEEYKLEITGTPSGHLTGYFTKE

General information: TITO was launched using: RESULT: Template: 3D33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 132 1857 14.07 32.58 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 14.07 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.635

(partial model without unconserved sides chains): PDB file : Tito_3D33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3d33-query.scw PDB file : Tito_Scwrl_3D33.pdb: