Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLDNSDKYLLPLLPKIILEKTNKK-AEKIKFIGGGSFGRVYKAELSNGETIAIKAYRI---QGSQYEEANQLNLLSKN--TSVKTPKVIFTYEDENTAILAMTFVEGKNVLNPLFLF---KSKKQKQLFANDVVDGMLQWHSVTGEKFGALSNPTYDSWYEYYKTVNQELWLKDLAKLAKNGKFSKKRMQLLYDATEIFNKL-PEETTAPVLIHSDLNIMNIMADPKTLKLTAFIDPCTSIWADREYDLFQLRNMW------GDAYGLYETYKKKYKLSKYADFRVAYYGAMLEASMRLKGGLI-LPFWEDLTMMRLKKEIKMLNNL 5IQI Chain:C ((9-300)) --------ATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN----NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLT---PEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID---NKQNVLEEYILLRET--I---YNDLT--DIEKDYIESFMERLNATTVF-EGKKCLCHNDFSCNHLLLDGN-NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNI--DIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYK--------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1213 31647 26.09 115.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 26.09 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: