Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLRNSDRTREEILTRYFPQY-RLIAPQAPSGLGGASCIIEQGEQRLVLRQNHDPLASSSHFRRQFRALKR-LPADL-VPKPRFYR-QGWMAVEYLAGE-IGNTL---PETAVLAAMLCHLHRQPRLGW-RVTLLPLLEQYWQQASADR---RTPFWLAQLKRLRKTGEPQPLRLSPLHMDVHAGNIVHTS-LGARLIDWEYAGDGDVALELAAVWSEDDAA---RQRLITDYARVAHMNADALRRQVRRWRPWVVMLMAGWFEMRFQQSRDKQFIALADDAWRQLQTKG 4R78 Chain:A ((21-296)) -----MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESD---QTPVSHEKIAIYKILQDTIWSLWTVYKEEQG--EDFGDYGVNRYQRA----

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1222 30930 25.31 118.96 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 25.31 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: