Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPNVKKGFFEAEVDGLKELSAF-VRVPDTYMLGETSEGAYLLMEWIE-PG--K--------GDQRDLAAALANLHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQEKYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQG--HPVFVDPA-VSYGNREQDIAMSQLFG------G----FRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF 4H05 Chain:B ((8-265)) -----LRGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAALGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRLDVAVALAGLARSLHALDWERCPFDRS--L------AVTVPQAARAVAE--GSVDLEDLDE-----ERKGWSGERLLAELERTRPA-DEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGWDGAVS-EEKLAFYRLLDE----------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1053 -9527 -9.05 -40.89 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -9.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: