Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVKTKLFYEDIKPFFDI---KSLQETKNGESHTVYILDN---DYILKIYEEE--NLFNIDAEIELLNYTKK---LCVPKVIKDDIF----------IKGKRGLVFSKAKGESVVEF-VKSTHLEQIGQFLNSFHKMTKNKKHDN-L--SAFGKS-------QLKVMIEKT--C---NKAFKDEFD----CLK-I--ELKNDGIIHGDLFLDNATFCGD---KLTCVFDFSDACEGDFIFDLAVVALSWCSN---KEEINILLKAYDKEIKL---D--DFIIYLRYASLYYCVKRHLTNRDYDNIIFKNKFIDLI 6EF6 Chain:A ((34-308)) -----------ALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVF------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 832 31148 37.44 143.54 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 37.44 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: