Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIIVEHHFAE-------LPKKINRITIGICNEVYRIALDD---------HDVIIRLSTSDK-YLRGSEVHIPEFKQLGIKVPDILASDYSQTRIPLCYQIQSKIEGQDLGVVIESLNQTELMGLAREIATIFSKVKTLPVTTQFGVIWGGDNDVSDSWAERMKLWIQESIKHGRLTGIMNQQFEGIAKDLYRNYETYFD-SIKPTMYY-GDICSKNVMINQG------VFNGLVDLDGLTQGDPLEALSRIKLSWYGT---------------SHGEFYYNALIKELE--L---------------TQSEQQLITVYALLNQLSWMCENGIQFNQNTKPIVNWEKAESDKNIYLQLASDLNNK 5FTG Chain:A ((12-350)) -AYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---EAMVLESVMFAILAERSLG-PKLYGIFP-------QGRLEQFIPSRRLDTEE--L---SLPDISAEIAEKMATFHGMKMPF-NKEPK-WLFGTMEKYLKEVLRIKFTEESRIKK---LHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAK-----------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 950 -36122 -38.02 -140.55 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -38.02 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: