Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSATTDRTQRPLLSKSKKLNSLHSKSSPALNFDESHSLSIKRRKVNTRDPAVYFVTIG--SKRMLLRIYGIGCEQILDRDKELDWLSRLSRLNIGPSLLGTFDNGRFEEYLESTTLTWHDLRDPFISAQIASRLNQLHSIVDTFPPAENEPLEVWQNIDKWYRSLESEVLSTLKKNPVWAKMIEQSLDLSQLHK--------DIETCKSILNTLSTPTVFAHNDTQYGNILKIENTD-----ELVVIDFEYAGYNPRGYDIANHFCEWMYDY-HSSEP-AKMNHKSYPTHKEQVRFLTAY------DKHHVT---------ELLREVELWKMACHLFWGLWGLVQASQSEIDFDYFGYSLERLSVFKQELDAKATQIA 2CKO Chain:B ((85-381)) ------------------------------------------------------ATLGDEPRKVLLRLYG---EAMV---LESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKM---PFNKEPKWLFGTMEKYLK----EVLRI--------KFTEES-RIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAY------------

General information: TITO was launched using: RESULT: Template: 2CKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 943 -6717 -7.12 -26.03 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -7.12 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2CKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2cko-query.scw PDB file : Tito_Scwrl_2CKO.pdb: