Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPDAWRRTFADAVIEQQTIGESRADVWRISPADSAA-LFVKCEQVMTWGELPGEVQRLRWLETLDLPAPRVLETTTEAEHNWLLMTAVPGRDLASSEQLSP---LQIVTLTAQALRELHAVAIDSCPFDHCLDKKIPLAREHVRAGLIDEEDFDDARLGRSAEDVFQQLLAERPPHEELVVTHGDACLPNLLAEHGRFSGFVDCGRLGVADRYQDLALAAHSITDNLGEQWLPAFFEAYGV-QPDQRRIAFYQLLDEFF 4FEX Chain:E ((31-272)) -----------------RDNVGQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTAF-MPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGE-FSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF

General information: TITO was launched using: RESULT: Template: 4FEX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1069 -24420 -22.84 -103.04 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.77 3D Compatibility (PKB) : -22.84 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_4FEX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fex-query.scw PDB file : Tito_Scwrl_4FEX.pdb: