Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLTEANVLDYLTGRGFAGPEFVVRGEWTVRNLSRRNRNFRVTRGGREFLVKQAGTWD-L----PGRASIEREADLCRRAATDPCFGALRPLVPDVYSYDPDHSILIFEFLPDEASLSDVPERL--DARTARLAGELMADYHRQMQSAALAEH-F-PGSLPGYF-SMHRW-DSERL---------VTRSQGQRELVRLVKRHAAFAPALESAAAE--WRPGALIHGDWKLENCLISNDGARMHVVDWELAGWGDARWDVATLLQSWWKRWVRDPA------EYRL----EAMRPELRALLNPS----------------EDEVAAILRFAAVRMLQSAWESLQ--DIPAIHGE------AVRMAQVSLNILTNPEWAGEAVFGHD 2PUL Chain:A ((12-382)) --LNESSAVALAVKLGL-------TLTCQEIGDGNLNYVFHIY--DRALIIKQAVPYAKVESWPLTIDRARIESSALIRQGEHV-----PHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAETLIHGDLHTGSIFASE--HETKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDGADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFK-------

General information: TITO was launched using: RESULT: Template: 2PUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 12358 8.75 40.65 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.75 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_2PUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2pul-query.scw PDB file : Tito_Scwrl_2PUL.pdb: