Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIESILKNIIKIEPITTGKSNDKKYLLVLKN--------GTLLFERVGPISRYDRFKMMYDELIDLKSLNEPGVPIPYFV-TKEDDTAVLLTSYSKGIPLNEVITDKNCSLDRVKELGRKAGNLLASVHTLSPLVPTD-SGDYDVLFKNILSDYVNLSLY----LK----H-EQDMISFVLNHMKEVM--TARPITYIHGNFDERILWVSPT------DRIFFVDTCDISSFDPFYDFKNIGVITR-----------------FENIEFAKSIIDSYTQGHP--------------TDEFWLAFELYNAFKLMSTTIELIKEGKEKEAIDKFETVADDFHGFKEDEIGKPKWY 5FTG Chain:A ((30-349)) --------DEFHISVIRGG-LSNMLFQCSLPDTTATLGDEPRKVLLRLYG----EAMVLESVMFAILA---ERSLG-PKLYGIFP-Q--GRLEQFIPSRRLDTEEL-------SLPDISAEIAEKMATFHGMKM-PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLR-SLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQA------------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 812 -734 -0.90 -3.09 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -0.90 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: