Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELGNFQKSVDSDEIKTMAFEIIGEPVKAVSRICEQWGEAEDEVREYDVYVIETDAGKYTLKKTGS---------KEAQIYAQYLSKGEFHVPQYVGMRQAEDADWICMKYVEGN-DMRDMT-DETTEKAAETLSKIQSYFWTPSMDKAPENEVEQRFVEYWKRVLRRASS---VADDPILRKAYQMFLDRQLTCPCTMSNGDFLQWNVIYDG-ENVVMIDWGFGGMMPYPLDIARFLAHATETRSTFPFYMNDAQKELFLDRMYEALKTKISREQFNLDVKLATLNEYIEFVEAEEDEDGWYLEHAQALAEELLNL 4R78 Chain:A ((21-252)) ----------MEKIIKEKISSLLS----QEEEVLSVE--QL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKD-LGLDVKNYLFDI---EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFA---PF-EE-IKKYESLIEEQIPYANYESVRNAVFSLEKRLA-DLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFT---------SQEEETFLSHYESDQ-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 902 8083 8.96 37.25 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.96 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: