Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIWGDGTFVMASGQLLCFARLFLLVPQLSSDAPDTILPDILEAFERHQDLHRVCGSPLSARPQPGLTNRVFRLEAEK---------GTFFLRLPRLEAAG-NIDRKAEARNLMLAAATGIAVPPVFCDAEAGILVTRAVKVSGSPAALSEQPSYSLARALGEALGQLHGSGVTFEGLLD-PDSVLQAQWQGLRDAVR-----CREEMTGL-EIVLQPI-GA-ATADGAGPALVPSHGDPSPGNCLMSC-------DQLWLIDWEFSGMSDPAWDLAYAVLEHEM------------------SADEEQVFLQAYAATGA--------------AVPEADRLCVMKAKCDAVSALWALEQVAAGRDEDVFLPFARQRRGRALRQLARLS 5FUT Chain:A ((32-376)) ---------------------------------------------------------FHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPSRRLDTE-EL-SLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKL-

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -5328 -5.75 -20.18 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -5.75 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: