Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence AGISNT---NPP---GGAPRPRQQCFLSAD--LGPDV-MGQPTLLEATPFVNDMFDFHGYRCQMGKDCSHVRCRGFPAAFKMTAR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1J2Z Chain:A ((2-260)) SKIAKTAIISPKAEINKGVEIGEFCVIGDGVKLDEGVKLHNNVTLQGHTFVGKNTEIFPF-AVLGTQPQDLKYKGEYSELIIGEDNLIREFCMINPGTEGGIKKTLIGDKNLLMAYVHVAHDCVIGSHCILANGVTLAGHIEIGDYVNIGGLTAIHQFVRIAKGCMIAGKSALGKDVPPYCTVEGNRAFIRGLNRHRMRQLLESKDIDFIYALYKRLFRPIPSLRESAKLELEEHANNPFVKEICSFILESSRGVAYKSS

General information: TITO was launched using: RESULT: Template: 1J2Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 10184 31.14 135.78 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 31.14 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_1J2Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j2z-query.scw PDB file : Tito_Scwrl_1J2Z.pdb: