Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------KQIAQLKEYLRNPPCKVTIYAPPLKQGQT-QLRTVMGFASAGKNEDVPVKIASTGHTYQCRTDATCEEPVCHGLPNGWTYEGVY 1PCH Chain:? ((1-88)) AKFSAIITDKVGLHARPASVLAKEASKFSSNITII----ANEKQGNLKSIMNVMAMAIKTGTEITIQADGNDADQAIQAIKQTMIDTALIQG-------

General information: TITO was launched using: RESULT: Template: 1PCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 297 21511 72.43 298.76 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 72.43 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_1PCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1pch-query.scw PDB file : Tito_Scwrl_1PCH.pdb: