Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTKTSHFSPLDFPEQLRTYIEGA-TLSDSSSHSGATVLYLDS-----GYYLKIDQ---KRRLERETAIARWFETKGLG-TPVIDYLS-TDKDYLLTKEAPGKDALAFLD----QPETICRTMAHALKKLHNLHPHNFPSENHLQTYKDRALKNYEKGEFYAKALLPQFQINSREEAFQLIQEQGHLLKTDAFIHGDACLPNFILKDASRFSCFIDLGLADFSDRHIDLFWAVWSLNYNLDNPKYAELFLDYYGREEVDTNKLRLVAAFEAFG 4GKH Chain:A ((19-272)) ---------------NLDADLYGYRWARDNVGQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTA-FMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEG-KLIGCIDVGRVGIADRYQDLAILWNCLGEF--SPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF

General information: TITO was launched using: RESULT: Template: 4GKH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1039 -6288 -6.05 -26.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -6.05 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4GKH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gkh-query.scw PDB file : Tito_Scwrl_4GKH.pdb: