Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSALELPAKILEWIEKAAGSPVLKADRIPGGGTRQGWLVD-VAAAAAGDGSGKLFLRYSPERLPDRGAFHRLATEAEVLRALRGAGVAVPAVCAVHPV--EEAVLLERVPGETWFYRIRDPDEQVRVAQDFIRSLAKVHRLDPADLGVP-GLGPVRSAREHALERIAEIRRRGTAPDGSIDPLVRLSADWLESHVPDYDGPVVLVQGDTGPGNFLYQDGRVTAVLDWELCHFGDPMDDIAWLSLRTVQDTFTHLPDRLAEYEKLSGHAIDVDRVRYYRVFAETTMATLTPNEGARAPAAEDGSGGADKDVGNHLLYAQLHRRLWLEALNAVMGLGLKKPVMPEPVDSSDWQPLYGDVLAMLRTITPRMGDALAVQWSKGIARLVRYLRELDACGRAGNELERSDIASLLGFTPLSLPAGRDALARANAAGRVSDVDFTRYLWNRVMRDDHLMRHASGALHSRTWPPLTNPS 1J7L Chain:A ((2-264)) --------------AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYK--GTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPE--EELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGE--EQYVELFFDLLGIKPDWEKIKYYILLDELF-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1J7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1220 11731 9.62 45.29 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 9.62 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.233

(partial model without unconserved sides chains): PDB file : Tito_1J7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1j7l-query.scw PDB file : Tito_Scwrl_1J7L.pdb: