Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIESILKNIIKIEPITTGKSNDKKYLLVLKN--------GTLLFERVGPISRY--DRFKMMYDELIDLKSLNEPGVPIPYFV-TKEDDTAVLLTSYSKGIPLNEVITDKNCSLDRVKELGRKAGNLLASVHTLSPLVPTD-SGDYDVLFKNILSDYVNLSLY----LK----H-EQDMISFVLNHMKEVM--TARPITYIHGNFDERILWVSPT------DRIFFVDTCDISSFDPFYDFKNIGVITR-----------------FENIEFAKSIIDSYTQGHP--------------TDEFWLAFELYNAFKLMSTTIELIKEGKEKEAIDKFETVADDFHGFKEDEIGKPKWY 5FUT Chain:A ((30-349)) --------DEFHISVIRGG-LSNMLFQCSLPDTTATLGDEPRKVLLRLYG----GAEAMVLESVMFAILA---ERSLG-PKLYGIFP-Q--GRLEQFIPSRRLDTEEL-------SLPDISAEIAEKMATFHGMKM-PFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLR-SLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQA------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 805 -9766 -12.13 -41.20 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -12.13 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: