Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------GECIVKLH-DPNGKVVSWFIALA-GKR---EVKKEAAFSWSCSTNSETCEAEIPLCIVPKNFKPVGEH- 1IHR Chain:A ((2-74)) ARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNI-----LFKINGTTE

General information: TITO was launched using: RESULT: Template: 1IHR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 90 9089 100.99 156.71 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 100.99 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1IHR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ihr-query.scw PDB file : Tito_Scwrl_1IHR.pdb: