Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------WDSPVTHREHRPAPSPPQRCKVE-FGRANKPIGQAIEYWDRDFIIQG---YTFHCYADCTLKGDYNFPRNQGYWVTS---------------------------------------------------------- 4F0D Chain:A ((2-271)) MGWAAAREAAGRDMLAADLRCSLFASALQSYKRDSVLRPFPASYARGDCKDFEALLADASKLPNLKELLQSSGDNHKRAWDLVSWILSSKVLTIHSAGKAEFEKIQKLTGAPHTPVPAPDFLFEIEYFDPANAKFYETKGERDLIYAFHGSRLENFHSIIHNGLHCHKTSLFGEGTYLTSDLSLALIYSPHGHGWQHSLLGPILSCVAVCEVIDHPDKYFVVTNNQLLRVKYLLVYSQ

General information: TITO was launched using: RESULT: Template: 4F0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 150 1798 11.98 24.62 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 11.98 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_4F0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4f0d-query.scw PDB file : Tito_Scwrl_4F0D.pdb: