Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAISRLLSEQLGE-GEIELRNELPGGEVHAAWHLRYAGHDFFVKCDEREL--LPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPR-PLDA----HSAFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAAEKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGP-DGPYIFDP-ACYWGDRECDLAMLPLH----TEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIAQQSLDRLLAA 4R7B Chain:A ((21-270)) MEKIIKEKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKD-LGLDVKNYLFDI---EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELR-GE-FA------P-F-EEIKKYESLIEEQ-----IPY-A----NYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTP--VSHEKIAIYKILQDTI-----------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 28257 28.63 119.73 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 28.63 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: