Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAFEKIPAFQQWQKVEAIHRGWSTDLKFKVTKNQETFLLR-IFQQEELLAKQQEYQFIKKVAALGFPSSKPFLCAPIPESEQGYMLLTYLEGEDLSDVLPALSPKR-QLNLGVEAGRYLNKIHKLLLPER--------IS-QREIAQNLYEKKQSQLNKYKESQFCMPYQQPIISYLEKQLPLLQQRPV-VYQHGDFHVGNFIYLP----TRQVGVIDFNRWDFGDPYEEFYKL------QFFSRNVSP-LFAYGQLQGYFAGKVPTLFWQFQKLYT-FHAGLYSLVWALSFGEKEIRTMEQQYQQLLEDYNCGELL-VPKWFSTI-------QRKGLRF 3CSV Chain:A ((4-333)) ----SREDEIRDFLATHGYADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPE----IYAEEHARGLLLIEDLGDALFTEVINNDPAQEMP--LYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQ---------LEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1338 1302 0.97 4.49 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 0.97 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: