Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQATLDWLAGLARQHPEVFGGHPGEMAVLEASRSSSHESRVYGS--G-GALVHVKFFRRTLGRTSVTYDPCREMKAEYDTLREFEKN-GFASGRHRVVRTLGINESL------DCALATAYVPGDTLLSIIQRTLKGGDDGELLEALGLAAGFLRKVHGLMPQSPKVD------AAETFYSYMKPLFYLEEQGALDGFHRRVTKGLSRWYGHRPLFEQRGVTVHGDANPSNFKIHE-G-VIYGLDLERSRPGRSPLLDVGTMVAELEHHCAYVAGDAGRAKPYVERFLRAYAPSPADRKKIDELLPFYVSRGLLKIAMLGYWKHGYRRSLVEAGARRIEEGP 5IGH Chain:A ((32-254)) ----------------------------------LGLDYRIVIATVDDGRRWVLRIPRRAE--------VSAKVEPEARVLAMLKNRLP-----FAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVV-------PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV---VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVD-DPAIDMAAHLMVFG-------------EEGLAKLLLTYEAA------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 776 17814 22.96 86.90 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 22.96 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: