Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTKTSHFSPLDFPEQLRTYIEGA-TLSDSSSHSGATVLYLDS---GYYLKIDQ------KRRLERETAIARWFETKGLG-TPVIDYLST-DKDYLLTKEAPGKDALAFL---DQPETICRTMAHALKKLHNLHPHNFPSENHLQTYKDRALKNYEKGEFYA--KALLPQFQINSREEAFQLIQEQGHLLKTDAFIHGDACLPNFILKDASRFSCFIDLGLADFSDRHIDLFWAVWSLNYNLDNPKYAELFLDYYGREEVDTNKLRLVAAFEAFG 3TM0 Chain:A ((4-262)) -----------RISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKT-EKPEEELVFSHGDLGDSNIFVKDG-KVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIK-PDWEKIKYYILLDEL-

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1098 22902 20.86 94.63 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 20.86 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: