Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGAGAADATEDAATDATRGTDATRGTDATGGTDGPVPAARTAEEDRLVARVEELVGG-------RVTAIERQ--PRWRKAWYLTVDRD-----GAELALYVRGDKQIDAEPYPGLDREAAILRILERNGVPVPHVHGMSSDPIGIIMDRVPGTRDVAEAADDAQRRGIAEQYMEILARMHSIDVAEFAAAGIEVPTTPEGAQLAFVDANERL---YRRTKK-----APEPLVEWALRWARRRLPTAGNRARFIHGDTG--QFLFVDGRITCVYDFEASHIGDPLSDLAGLRTRAGTE--------------PL-G-ADIEHMIRHYQRVAGTT----------IDPSALSFYTATYMLTAVMALSGPLTELRPADQQAEYLTWDLMVRRAMLWAIAEVEGVKIEPAPPVTLPTGYPARVTTVLEGTVRRMVPATGVDEANQSAALALAQWAGAMVAVGMANVERDLDRAAVLLGHRPADQAAADAELERFVLAAGPEHDLALLEYFAAQTEARVAEAVPMRTRLEQYALPKVVL 1NW1 Chain:B ((51-413)) ------------------------------------------ELKERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPET--ESHLVAESVIFTLLSERHLG-PKLYGIFS--GGRLEEYIPSRPLSCHEI---SLAHMSTKIAKRVAKVHQLEVPIWKEPDY--LCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLS----------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1165 10198 8.75 36.03 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : 8.75 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: