Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASDRQDLSDRIARAIGH--GVRRLSPLSGGCVGDVLRAELEDGAAPVVVKVAD-DAGGGLDIEGWMLRYLADTSRLPVPGVLHAEA----GLLVMEEIQAGGGGISASCQEHAADLLADLHSVTWD-AFGLERDTLIGGLHQPNPREASWRTFFRDHRLLYMADESHRAGRLPADVRRKVDTLAGRLDDWIE-EPDAPALIHGDMWTGNVLCDGGRIAGFIDPAIYYGDAEIELAFSTLFGTFGEPFFRRYQEKRPLRPGFFEARRDLYNLYPLLVHVRLFG---GSYVGSVSRTLDRFVG 3JR1 Chain:A ((19-306)) --GMWKSISQVLAEQFGAYYFIKHKEKLYSGEMNEIWLINDE-VQ-TVFVKINERSYRSMFRAEADQLALLAKTNSINVPLVYGIGNSQGHSFLLLEALNKSK--NKQSSFTIFAEKIAQLHQIQGPDKYGLDFDTWLGPIYQPNDWQTSWAKFFSENRIGWQLQICKEKGLI----FGNIDLIVQIVADTLSKHNPKPSILHGNLWIENCIQVDDK-IFVCNPACYWGDRECDIAFSSLFEPFPTNFYQRYNEIYPLEEG-YLERKLIYQLYYLLNFSYRYYNKKQSYVSLTQKLINQI--

General information: TITO was launched using: RESULT: Template: 3JR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 -23738 -17.44 -86.01 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -17.44 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_3JR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3jr1-query.scw PDB file : Tito_Scwrl_3JR1.pdb: