Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTVAAPAPALAEVACFSRDLCKE-LVKEWSSLDSSCFSISTVSGGITNMLLKVSVKEGS----DSESSVTVRLYGPNTDLVIDRERELQGSGLGYLEYLRMEWFRHSSMLGHYHLQVYTQRLL--FCTHRCMETGEPAINASANNMKEPRIAAEIAKQLQKFHQVDIPGSKEPQ-LWNDIFKFLKKASVLKFEDNEKQKRYETISFREIQDEVKELKDLSDLLHAPVVFAHNDLLSGNLML-----NDLEEKLYFIDFEYGSYSYRGFDIANHFNE---------YAGFECDYNLYPDKDVQYHFFRNYLSDRPSELKNRLFASICEKRNVGYLAFVQYKVQEQDLDDLYTETNTFRLASHLYWALWALIQARVSPIDFDYLGYFFLRRHVVPNRKLYNL 5EQP Chain:B ((39-384)) ---------------------CKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------------------MVLESVMFAILAERS-LGPKLYGIFPQGRLEQFIPSRRLDTEELSL---------PDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFEN---LSTEEKSIIK--------------EEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDY----------------

General information: TITO was launched using: RESULT: Template: 5EQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1201 21380 17.80 71.99 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 17.80 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_5EQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5eqp-query.scw PDB file : Tito_Scwrl_5EQP.pdb: