Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTSSQLNSLRSLPCFEKVVDISLLIDGMSHTCIKVTTA--LQVFFVKKLNSET--AETEVLSSKYAAKHGLSPRIIYHDNAWLVTDYIVGITLDKNE-LSSSQCIDTGLTLMAKLHQLVPQPNAKTI-------PCLDTSESVRRLFTNPNSIPSQQKL----------F----LADITETLTLKIGIEQKRSDVTNIICHGDINYSNIIIDE-TRQSWLIDFECSHLAPVDFDLSMFIAINN---------ISPLHIDEIVNRYMSLVPSY--KPNKILLTYYLLYSYFINGLWYLDNTNDAKINNHLRSLAIEQWSAFDSFVVKYHLDIPKLMPLIS 2QG7 Chain:D ((110-453)) -------------------SLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTDNREREKKISCILYNKNIAKKIYVFFTNGRIEEFMDGYALSREDIKNPKFQKLIAKNL-KLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESL-CKRENSPIVLCHCDLLSSNIINTVG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-DFDFINYGMTRLTASCLPI---------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1080 -8095 -7.50 -30.66 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -7.50 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: