Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEKDRAVVTELLQKAGREDFIPLQENQLAGDGSSRRFYRLASG-GETLIAVLPAGEEAKDRAESRAAYHIGGYLLACGTPVPAIYGWD-------EASGGLLFQDLGNTRLQDVAHTEEGRQLYRQLVRALAGMNCRARGIDSSWCWDSPCYDRSLMLERES-GYFLRAFWQGLLGQEVPIGLAEEFSSLADAV----SLYSTDFFLHRDFQSRNIMIHAGRPSIIDFQGGRRGPLGYDLASLLYDPYVDLDEDFRALLWEDYLAALGAQIDISREDFAKQYSLLACQRSLQIIGAFSWLSAVAKKTFFVQFIRPSLLNLEKILSAPYYQNYRVLRQTVRRGLSLYR 1ZYL Chain:A ((10-280)) --TLHPDTIMDALFEHGIR--VDSGLTPL-NSYEN-RVYQFQDEDRRRFVVKFYRPERW-TADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEAD-----NIDQMEAVGRYLGRMHQTGRKQLFIH--RPTIGLNEYL--IEPRKLFED-A-TL-IPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKA-EQRMQLETIIEAYEEFS----EFDTAEI-GLIEPLRAMRLVYYLAW---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1122 -17729 -15.80 -68.72 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -15.80 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: