Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNLDGLLSR--IQ-AKTFSLVTHRDGATVYKAQTTQ-GYVFVKMYTRK---VDHERYCEILDELRA--FSLVPKLLNQFEW-ENYFV----VVMSALEGQPVNNILKNCSRTEKLSLLKEAGASLGSFHRAISPSRLFEMKFWKGRDDS-SKDPTLWNEHLKLMTTKWMSRIDPTKADYKEFSYELSELALYCKD-LREPTQFSLLHCDYIGRNLLATSC-NKISGVLDYEAARIGDAVYDLAKIVWLDMEFSDLELRHAFLQGWEKT-YG-EVVPQREFLTYVGIQCLAAIAWTDKHAPIEGNTIFRASAIKALKIVANEL 5IGH Chain:A ((12-297)) ---LYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAV--------DAGMLIRTPTQARQKVADDVDR----VRREFV--VN-DKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLID-NTERVSGMIDWSEARVDDPAIDMAAH-LMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSG----NEEYLAAAKAQL-------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1194 -1045 -0.87 -3.91 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -0.87 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: