Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSSAPYNEQVIRDITKILNANFSFPT--SITNIELLVKGM-NYTLRVECKNTKYYLKIFSASRDK-I-DI-SFEMHVINRLSASGIGVATAINSINGDAYINLTLAGEDRLAVLYAHVGGRTLT---SDPRDIFLFSKALAEIHRTPTSFFLQYT-----SRIFSI---ESECYRIRSS-IEK---HSYIPKPLASKLLDNCYSIQALASTDC-AVLSHGDAWLGNAKYV-N--GVIYFIDLEDSKLANRNFDLGVMAYNLIVKK----------YEVRENIQALLRGYNE--LNTSIT-SGSIKPY-IQLRSLFVLCFLLENKLVDQSLLQKVFERAEYFTSSKFDSEMAHLP 4OCV Chain:A ((26-321)) ---------------EALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTM----------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1053 17903 17.00 69.66 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 17.00 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: