Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCYGSMSCNPVEQRGEELKYMEKLIELYAQWAGEKP-STIEKLEGAGSNRVYYRFGNKSGQSVIAVIGTS----RDENHAFIYLTEHFTD-KQLPVPQILAVSDDELRYLQTD---LGHTSLFDMLKSARDAGGRYTQKEKQLLTKTLQALSEMQVRGAVGLDFQQCYPQPEFNVESVLFDLNYFKYCFLKATGIDFHELMLEA---NFRMFAKDLT-AEPAECFLYRDFQARNVMLDND---GNPYFIDYQGGRKGPYYYDLASFLWQASA-KYPFKLRRDLVSAYYESLKQYTEVPSSRHFVERLNLFVLFRTLQVLGAYGFRGYFERKKHFIQSIPAAMQNLRELIKLGEHVFPYPYMMSVLKEMTELPQFANKEERKVKRTDGFKITDNNIFKANPVDGPATFSKYDDKGELIVRVNSFSYHKGIPEDPTGNGGGYVFDCRSTHNPGRYEPYKNLTGLDEPVIRFLEDDGEILVFLESVYQLADRHVARYIERGFTSLIFSFGCTGGQHRSVYCAQHLAEHIHDKFGIEVRINHREQKIEQTLEAK 6EF6 Chain:A ((27-321)) --------------------DVTIAQQALTHYDVSDNASLRLL-NLSENATYL-VEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEE---SLTLDDFQTLGRITASLHEHSQR-WTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRE-----LPAADE-AMLPSFVFLRRLLLLAWMGSH---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1274 5758 4.52 20.94 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 4.52 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: