Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSDETHAYWLEAAHALLGDA-SAEILAGDSEVATLL-LTLTDRRQVVMKAVRAASEKRANLFFQWEALMLLSAEPDFPGPQPVSRHSLSDETEVFVLGYVPGRHPAFE-------SDEDFRVFGATLARFHKISKGKRLS-GATNWDLHRVARHYENPLLLQLLSDQERTIVAAALDRFGPQFQAQIDEGVWTGLVHSDSHRHNVVIDGA-----RGSLIDFGECGFGALFWDLGVAVADSAVDAPERGEVCRQNLVAGYCTVTPEAEPIVDKTLPVFEAMRSLEVITWPVSDWSPERLAGDKEEARDNIEVSVRHLEALLDSP 3CSV Chain:A ((11-307)) -----------DFLATHGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEE--GGDTQPFVDLAQYLRNL-DISAPEIYAEE---HARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETL-SEMTRLAFSEYRYA-ILGDAAEDNRKRFEHRFAQILSAQ-LEGDM-VFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARR--D-VPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRF----GKRHYIEFVPRVWAHFERGLAHP

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1246 1506 1.21 5.50 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 1.21 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: