Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTT--NKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI--KTKFDKITPILQTIHTSAKELRG--------------EFAPFEEIKKYESLIEEQIPY------------ANY-ESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLES---------EFTSQEEETFLSHYESDQTP----VSHEKIAIYKILQDTIWSLWTVYKEEQG--EDFGDYGVNRYQRAVKGLASYGGSDEK 2QG7 Chain:D ((110-454)) -----------------SLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTD---NREREKKISCILYNKNIAKKIYVFFT--NGRIEEFMDGY-ALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLPIF--------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1010 21186 20.98 82.43 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : 20.98 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: