Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MIESQAPMLEMLWEPHDPRHALAERFGFS--DSESASRWVAAM----LDEHWGIRIDSCERILMSGGNALAWVGTPSGR---LLAKWS-VVLDCFPRLME---TARLTNWLHGRGLPVSVPVPTRDGCLQVEVDRVSMGLQREI-----VGDLLDTGDLNQVRAAGVILAQLQDALAAYPDADQFL-----------APVVSSKPLTVRVTDWLDSRAD-------------HLPMAARDTLRGLVASAPPDRLPRQLVHFDIRSANILWACGGVAAILDFEEAQHDHR---------IVEL-ARAAVLLGTRYHNWGPVSADVRTEFLTGYQSERL--LTPAEAGWLHIVLLW-------QALAMVP-PGDDPTGWG--PSALSQLSQETAR---------------------------------- 4P0G Chain:A ((7-435)) APTALERVQKEGVLRVITRNSPATYFQDRNGETGFEYELAKRFAERLGVELKIETADNLDDLYAQLSREGGPALAAAGLTPGREDDASVRYSHTYLDVTPQIIYRNGQQRPTRPEDLVGKRIMVLKGSSHAEQLAELKKQYPELKYEESDAVEVVDLLRMVDVGDIDLTLVDSNELAMNQVYFPNVRVAFDFGEARGLAWALPGGDDDSLMNEVNAFLDQAKKEGLLQRLKDRYYGHVDVLGYVGAYTFAQHLQQ-RLPRYESHFK-QSGKQLDTDWRLLAAIGYQESLWQPGATSKTGVRGLMMLTNRTAQAMGVSNRLDPKQSIQGGSKYFVQIRSELPESIKEPDRSWFALAAYNIGGAHLEDARKMAEKEGLNPNKWLDVKKMLPRLAQKQWYAKTRYGYARGGETVHFVQNVRRYYDILTWVTQPQ

General information: TITO was launched using: RESULT: Template: 4P0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1678 38457 22.92 117.60 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 22.92 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_4P0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4p0g-query.scw PDB file : Tito_Scwrl_4P0G.pdb: