Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVARTINLIEEEIINDLKGKFGWHIQSLEANNLGYGNMKWIMKTD----SESYFVKKYCKVRYRR-----GLEDVHE-----------ALRYQDLMYRDGI---PCQPVYAYNN-----RYIHRLQSDEDYMIT-GVSKGELIQA--GTANLQQMYSL-GETTGRMHKWMSIHMPRRTVLHWDLPSKHKMIARLELNLHETEEANHGKYVLAIQKQMEILSGVDMDI-FNECAKGWAHWDMHVDNLLFYK----------DGVADIVDFDRVQFVYPDFDISRALLSCALKDDQMRLDAVEAYVEGYRVHMSLSNEQLVRSIKLTWYKEFKWVHAMYCSEKTMGRFIDEMI-----WIGDNWNELNDIFKL 5JDA Chain:A ((1-359)) -SMLPSYDFFIHPMNLVELKKDIWSDSPVPAK-LTYGKKKYDIDIVYRGAHIREFEKKSYHVMFYKPKKFQGAKEFHLNSEFMDPSLIRNKLSLDFFHDIGVLSPKSQHVFIKINGQIQGVYLQLESVDENFLKNRGLPSGSIYYAIDDDANFSLMSERDKDVKTELFAGYEFKYSNEN----SEEQLSEFVFQANALSREAYEKEIGKF-LHVDKYLRWLAGVIFTQNFDGFVHNYALYHNDETNLFEVIPWDYDATWGRDVQGRPLNHEYIRIQGYN-TLSARLLDIPIFRKQYRSILEEILAEQFTVSFMMPKVESLCESIRPYLLQDPYMKEKLETFDQEADMIEEYINKRRKYIQDHLHELD-----

General information: TITO was launched using: RESULT: Template: 5JDA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1561 4618 2.96 14.85 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 2.96 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.152

(partial model without unconserved sides chains): PDB file : Tito_5JDA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5jda-query.scw PDB file : Tito_Scwrl_5JDA.pdb: