Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSASPSLQVTAFEGNRCIASGPLASVAEKIKEVLARGEAASVLVFDDQTGSQVDLNLRTLSEA--AQGMPT-PL-IAPEPLESRNAGRPRLGVVAREVTLLPRQWEWLGQ----QQGGASATLRKLVDQARRENAGEEKIRAAREALYRFMTAMAGNAPGYEEALRALFAGDVHRFSILTSDWATDVRKHVGKLAPAAFGYAPSPLDVVIPLTKREAVLRAFGTAEVQSIERITNGASGAGVFKVK-ANQVDYLL-RI-EGPPDGLRDPARQYACLKIAAEAGVAPRLVYADAESGVAVTDFIAPDSASVERSKADSLRRIVTAVRSLH-DAPLFPELVDYLDG-VDTLIR-SCMETGILPKRAIETHRKFYGELAAAYPRKNLDLVSSHNDLNPGNVLFQKERVWLVDWESAFAADRYVDLAAIANFFTTEENEKELVLESYFGAALNDLHRARFFLMQQANRMFYAMVVLNFVAAAEPAIKLTAAGMKGIRWSEVRGEMARINTTETKVRFAGALLNEALLAFQSPRFSQAVAQVKGSSKRRGA 5THY Chain:A ((19-405)) YFQSNALPPDFLLD------------PVEVSQQLAPSLTELVTLLDNARTSEIGTQLEELSVDYIVQGLLQMGWSYQPTESFDLDAAAQCLGVVPTQVRLFERLLQILAEVGILQSNQQQWQVQK-TAQKVNPSKQSQSLLSQYPDEAATLTLLERCASQLSGVLR-------------------------GEIDPVQLVFPQGDLTTATQLYKDSAVAKV-------------------------MNTIVEKVIMKAMEKLP-----PSRGIRLLEIGAGTGGTTSYILPHLNPNQ--TEYIFTDIGALFTSKAQEKFQDYRFLGYQTLDIEVDPSSQGFESHRYDVIIAANVLHATTSLKQTLSHVRQL---------------------LAPGGILVLYEAT---------TRSRWVDLI-------------FGLLEGWW--KFTDYELRPDYPLLNREQWKKVLSETGFT--------------QVVTLPEVEGM-------------AEALSQQTVIVAQAAS-----------------

General information: TITO was launched using: RESULT: Template: 5THY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1863 25458 13.66 68.25 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 13.66 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.128

(partial model without unconserved sides chains): PDB file : Tito_5THY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5thy-query.scw PDB file : Tito_Scwrl_5THY.pdb: