Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNREEACRVVTVPESMSAALAGYAWARDRVGESGGAVYRLHGKPGAPDLYLKHGRHAVADAIGDEMTRLRWLAGRMPVPAVVHFDRSADEAWLLMTAVPGRTAYQLLQADPAQHDIVVDALARFLQRLHAIPTSACPFNSDHDYRLGLARSRIEAGLVDEDDFDEERAGWTARQVWDAMQALLPLAPDPVVTHGDYSLDNLLIVDGQVAGCIDAGRVGIADRYQDLAIAWNCLGEFGGSLQERFLRQYGIAAPDRRKLQFHLMLDELF 4FEV Chain:F ((17-272)) ------------NSNLDADLYGYRWARD----SGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF

General information: TITO was launched using: RESULT: Template: 4FEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1156 -143014 -123.71 -567.52 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain F : 0.88 3D Compatibility (PKB) : -123.71 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4FEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fev-query.scw PDB file : Tito_Scwrl_4FEV.pdb: