Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPSMCRLMPVSFRIRIYHFLSSLGSRVYGSSCSLRVQQL---PFGVYLKTKSVE---DHQALKIEFGALQLVRSQTQIPVPRPLDLLSDTETSYLLTTTLPGQRFGSYIDILSDHDLDLFTRDMQRYLTQLRSISRPRTPKYEIRNAIG-GPYYDYRIIAAHDY-DKECGDFFGPCTDEEDFNNILRTPALPYVFHSTGHNIVFTHSDINMRNVLMHNGRISGIADRENSGWFSDHWEYTKAHYVTKLHKRWLAVVNRIFESFGDFTLDLEIERRLWEYCF 1L8T Chain:A ((23-246)) -----------------------------EGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG--KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED--EQSPEKIIELYAECIRLFHSIDISDCP--YTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKT-------EKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI----------------

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 932 46139 49.50 213.60 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 49.50 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: