Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVYTQLTDDQFSAFCQRFGVSF-ACAIPITQGIKNSNWFIQTTDDVDGAHSYVFTLFEER--PLEDIEK-MAVILNQLDGKLPVAAPLSLVDSAEKCYVIRYDNKAITLVPCLAGSHPQQTTQAMCHEIGTALAMLHETLQALQPSEQYGVPLY-PWSDVRDRER--QFMPADEAKLMSDIWQSYTDL----PLATLPKGLCHLDMFADNTLWNLSLNNSQKGEESLTGLLDFTEVSVEHYVMDIAITINDFCTTWGDAEQGESVNFDRSKMAAFLQGYESKRSLGADEKRALPVMLAKAAVIFWLLRLNVIHYNRTEGRTGDNIMVKNPDLMKRLAAYHWSHVEKAKNTVFVLLDNLLASQKDIQIVGVFSNITQAEQARDKLSNSSEFAIKEYTVDQLS 1ZYL Chain:A ((7-275)) --TFQTLHPDTIMDALFEHGIRVDSGLTPL-NSYENRVYQFQDED---R-RRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVA-F-NGQTL--LNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD----------G-PMFVDLDDARNGPAVQDLWMLLNGDKA------------EQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLV---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1111 -17808 -16.03 -69.02 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -16.03 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: